Equilibrium Studies of Kinesin-Nucleotide Intermediates
نویسندگان
چکیده
منابع مشابه
Nucleotide-free Kinesin Hydrolyzes
Bovine brain kinesin binds ADP tightly and contains a stoichiometric amount of ADP at its active site when isolated in the presence of free M 8 + (Hackney, D. D. (1988) Proc. Natl. Acad. Sei. U. S. A. 85,6314-6318). EDTA in excess of M S + weakens ADP binding and nucleotide-free kinesin can be prepared by gel filtration with excess EDTA. On addition of ATP, this nucleotide-free enzymle catalyze...
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O u r recent experim ents on the m olten globule state and o ther protein folding in term ediates lead to following conclusions: (i) the m olten globule is separated by in tram olecular first-order phase transitions from the native and unfolded states and therefore is a specific therm odynam ic state of protein molecules; (ii) the novel equilibrium folding in term ediate (the ‘pre-m olten g lob...
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Kinesin-1 is an ATP-dependent motor protein that moves towards microtubules (+)-ends. Whereas structures of isolated ADP-kinesin and of complexes with tubulin of apo-kinesin and of ATP-like-kinesin are available, structural data on apo-kinesin-1 in the absence of tubulin are still missing, leaving the role of nucleotide release in the structural cycle unsettled. Here, we identified mutations in...
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The neck region of kinesin constitutes a key component in the enzyme's walking mechanism. Here we applied cryoelectron microscopy and image reconstruction to investigate the location of the kinesin neck in dimeric and monomeric constructs complexed to microtubules. To this end we enhanced the visibility of this region by engineering an SH3 domain into the transition between neck linker and neck...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1996
ISSN: 0021-9258
DOI: 10.1074/jbc.271.16.9473